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A Shiny/R app to display the Illumina human body map dataset. I have not included the expression data due to it's size (19Gb) but this should work with any RNA-seq data set analysed with a TopHat->Cufflinks->CuffDiff pipeline.
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library(shiny) | |
library(deSolve) | |
library(cummeRbund) | |
# load cuffDiff data, must be in top dir and called cuffData.db: | |
cuff = readCufflinks() | |
shinyServer(function(input,output) { | |
getData = reactive(function() { | |
if (!nzchar(input$gene)) | |
return() | |
return(getGene(cuff,input$gene)) | |
}) | |
output$genePlot = reactivePlot(function() { | |
myGene = getData() | |
#fudge to stop shiny trying to plot NULL, which results in an ugly black square: | |
if (is.null(myGene)) | |
return(plot(1,type="n",bty="n",yaxt="n",xaxt="n",ylab="",xlab="")) | |
x = data.frame( | |
tissue = fpkm(myGene)$sample_name, | |
fpkm=fpkm(myGene)$fpkm | |
) | |
print(ggplot(x,aes(fill=tissue,x=tissue,y=fpkm)) + geom_bar(position="dodge", stat="identity") + | |
labs(title=annotation(myGene)$gene_short_name)) | |
}) | |
output$isoformPlot = reactivePlot(function() { | |
myGene = getData() | |
if (is.null(myGene)) | |
return(plot(1,type="n",bty="n",yaxt="n",xaxt="n",ylab="",xlab="")) | |
trackList=list() | |
myStart=min(features(myGene)$start) | |
myEnd=max(features(myGene)$end) | |
myChr=unique(features(myGene)$seqnames) | |
genome='hg19' | |
ideoTrack = IdeogramTrack(genome = genome, chromosome = myChr) | |
axtrack=GenomeAxisTrack() | |
genetrack=makeGeneRegionTrack(myGene) | |
biomTrack=BiomartGeneRegionTrack(genome=genome,chromosome=as.character(myChr), | |
start=myStart,end=myEnd,name="ENSEMBL",showId=T) | |
trackList=c(trackList,ideoTrack,axtrack,genetrack,biomTrack) | |
plotTracks(trackList,from=myStart-5000,to=myEnd+5000) | |
}) | |
output$tissueIsoformPlot = reactivePlot(function() { | |
myGene = getData() | |
if (is.null(myGene)) | |
return(plot(1,type="n",bty="n",yaxt="n",xaxt="n",ylab="",xlab="")) | |
x = data.frame( | |
isoform = fpkm(isoforms(myGene))[fpkm(isoforms(myGene))$sample_name==input$tissue,]$isoform_id, | |
fpkm=fpkm(isoforms(myGene))[fpkm(isoforms(myGene))$sample_name==input$tissue,]$fpkm | |
) | |
print(ggplot(x,aes(fill=isoform,x=isoform,y=fpkm)) + geom_bar(position="dodge", stat="identity") + | |
labs(title=input$tissue) + opts(axis.text.x=theme_text(angle=90, hjust=1))) | |
}) | |
}) |
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library(shiny) | |
# Define UI | |
shinyUI(pageWithSidebar( | |
# Application title | |
headerPanel("Human Body Map"), | |
sidebarPanel( | |
textInput("gene", label="Gene", value = ""), | |
submitButton("Submit"), | |
conditionalPanel(condition="input.gene.length > 0", | |
br(), | |
selectInput("tissue", "Select a tissue to view isoform expression in:", | |
list("adipose" = "adipose", | |
"adrenal" = "adrenal", | |
"brain" = "brain", | |
"breast" = "breast", | |
"colon" = "colon", | |
"heart" = "heart", | |
"kidney" = "kidney", | |
"liver" = "liver", | |
"lung" = "lung", | |
"lymph" = "lymph", | |
"lymphocyte" = "lymphocyte", | |
"muscle" = "muscle", | |
"ovary" = "ovary", | |
"prostate" = "prostate", | |
"testes" = "testes", | |
"thyroid" = "thyroid") | |
) | |
) | |
), | |
mainPanel( | |
conditionalPanel(condition="$('html').hasClass('shiny-busy')",img(src="http://i1215.photobucket.com/albums/cc508/nawel12/loading.gif")), | |
plotOutput("genePlot"), | |
plotOutput("isoformPlot"), | |
plotOutput("tissueIsoformPlot") | |
) | |
)) |
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