Python Tkinterで点滅させる方法 https://qiita.com/Ovis/questions/11f541611185d70c8925#answer-4c75d28128f6849cb266

blink.py

import tkinter as tk
import math
import pyautogui

info = {
    'position': (-1, -1),
    'state': tk.NORMAL,
}


def triangle(x, y, edge, angle):
    ok = edge / 2 / (3**0.5)
    r = math.radians(angle)
    sin_r, cos_r = math.sin(r), math.cos(r)

    x1, y1 = -edge/2, ok-100
    x2, y2 = edge/2, ok-100
    x3, y3 = 0, -120

    def rotate(px, py):
        return ((px * cos_r - py * sin_r) + x,
                (px * sin_r + py * cos_r) + y)

    canvas.create_polygon(
        *rotate(x1, y1),
        *rotate(x2, y2),
        *rotate(x3, y3),
        fill="#ff0000",
        state=info['state'],
        tags="triangle")


def stalker():
    """追従"""
    position = pyautogui.position()
    if info['position'] != position:
        x, y = info['position'] = position
        canvas.delete("all")
        y -= 50
        edge = 100
        triangle(x, y, edge, 0)
        triangle(x, y, edge, 90)
        triangle(x, y, edge, 180)
        triangle(x, y, edge, 270)
    canvas.after(50, stalker)


def blink():
    """点滅"""
    info['state'] = tk.HIDDEN if info['state'] == tk.NORMAL else tk.NORMAL
    canvas.itemconfigure('triangle', state=info['state'])
    canvas.after(500, blink)  # 500ミリ秒間隔、1秒間に1周期、1ヘルツ


root = tk.Tk()
root.geometry("1920x1080")

canvas = tk.Canvas(root, background="#fff", width=1920, height=1080)
canvas.pack()

stalker()
canvas.after(300, blink)

root.mainloop()