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# A function to extract reads from a stranded RNA-seq BAM file. Intended to be used as a custom importFunction | |
# in the DataTrack constructor of Bioconductor's Gviz package. With properly stranded data this allows the plotting | |
# of coverage tracks from each strand of the genome. | |
# | |
# 6 Nov 2014 - bensidders at gmail dot com | |
# | |
# UPDATE: 10 Sep 2015: | |
# The funciton can now plot data from libraries created in either orientation. Due to the manner in which Gviz calls | |
# this function, you need to specify the library orientation with a global variable "libType" to be one of | |
# "fr-firststrand" or "fr-secondstrand". |
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library(shiny) | |
library(deSolve) | |
library(cummeRbund) | |
# load cuffDiff data, must be in top dir and called cuffData.db: | |
cuff = readCufflinks() | |
shinyServer(function(input,output) { | |
getData = reactive(function() { |