Generate an ISO image/file using Python on Windows

generate-iso-image.py

# pip install pycdlib

try:
    from cStringIO import StringIO as BytesIO
except ImportError:
    from io import BytesIO

import pycdlib

iso = pycdlib.PyCdlib()
iso.new(interchange_level=4)

targetfilenameFirst = 'YourFile'
targetFilenameExt = 'exe'

targetfilename = '{}.{}'.format(targetfilenameFirst, targetFilenameExt)
targetfilehandle = open(targetfilename, 'rb')
targetfilebody = targetfilehandle.read()

iso.add_fp(BytesIO(targetfilebody), len(targetfilebody), '/' + targetfilename + ';1')

iso.write('{}.iso'.format(targetfilenameFirst))
iso.close()

targetfilehandle.close()