Read a .wav audio file, create the spectrogram, run ISA for components.

wav_components.py

import wave, struct, numpy as np, matplotlib.mlab as mlab, pylab as pl
def wavToArr(wavefile):
    w = wave.open(wavefile,"rb")
    p = w.getparams()
    s = w.readframes(p[3])
    w.close()
    sd = np.fromstring(s, np.int16)
    return sd,p

def wavToSpec(wavefile,log=False,norm=False):
    wavArr,wavParams = wavToArr(wavefile)
    print wavParams
    return  mlab.specgram(wavArr, NFFT=256,Fs=wavParams[2],detrend=detrend_mean,window=window_hanning,noverlap=128,sides='onesided',scale_by_freq=True)

wavArr,wavParams = wavToArr("bat_speech.wav")
hf = pl.figure(); ax=hf.add_subplot(1,1,1)
ax.plot(wavArr)

Pxx, freqs, bins = wavToSpec("bat_speech.wav")
Pxx += 0.0001
freqs += (len(wavArr) / wavParams[2]) / 2.
hf=pl.figure(figsize=(12,12));
ax = hf.add_subplot(2,1,1);
#plot spectrogram as decibals
hm = ax.imshow(10*np.log10(Pxx),interpolation='nearest',origin='lower',aspect='auto')
hf.colorbar(hm)
ylcnt = len(ax.get_yticklabels())
ycnt = len(freqs)
ylstep = int(ycnt / ylcnt)
ax.set_yticklabels([ int(freqs[f]) for f in xrange(0,ycnt,ylstep) ])

from sklearn.decomposition import PCA, FastICA
ncomps = 7
# reduce dimensionality with PCA
pca = PCA(n_components=ncomps)
y = Pxx.copy().T
pc = pca.fit(y).transform(y)
# run ICA 
ica = FastICA(n_components=ncomps,random_state=42)
z = ica.fit(pc).transform(pc).T
hf = pl.figure()
for p in xrange(ncomps):
    ax = hf.add_subplot(ncomps,1,p+1)
    ax.plot(z[p])    

In my answer to http://dsp.stackexchange.com/questions/10767/audio-signal-separation-identifying-interest-points/10784#10784