This file is an example of a simple bacterial incubation and OD600 experiment documented in the Autoprotocol JSON data standard.

biocoder_autoprotocol_oct_2015.json

{
  "refs": {
    "growth_plate": {
      "id": "3208kfhf394",
      "store": {
        "where": "cold_4"
      }
    },
    "DH5a": {
      "discard": true,
      "id": "3209xifd029"
    }
  },
  "instructions": [
    {
      "reagent": "lb-broth-noAB",
      "object": "growth_plate",
      "columns": [
        {
          "column": "0",
          "volume": "150:microliter"
        }
      ],
      "op": "dispense"
    },
    {
      "groups": [
        {
          "transfer": [
            {
              "volume": "2.0:microliter",
              "to": "growth_plate/0",
              "from": "DH5a/0"
            }
          ]
        },
        {
          "transfer": [
            {
              "volume": "2.0:microliter",
              "to": "growth_plate/12",
              "from": "DH5a/0"
            }
          ]
        },
        {
          "transfer": [
            {
              "volume": "2.0:microliter",
              "to": "growth_plate/24",
              "from": "DH5a/0"
            }
          ]
        },
        {
          "transfer": [
            {
              "volume": "2.0:microliter",
              "to": "growth_plate/36",
              "from": "DH5a/0"
            }
          ]
        }
      ],
      "op": "pipette"
    },
    {
      "lid": "low_evaporation",
      "object": "growth_plate",
      "op": "cover"
    },
    {
      "where": "warm_37",
      "object": "growth_plate",
      "co2_percent": 0,
      "duration": "12:hour",
      "shaking": true,
      "op": "incubate"
    },
    {
      "dataref": "OD600",
      "object": "growth_plate",
      "wells": ["A1", "B1", "C1", "D1", "E1", "F1", "G1", "H1"],
      "num_flashes": 25,
      "wavelength": "600:nanometer",
      "op": "absorbance"
    }
  ]
}

Generated from this code https://gist.github.com/bmiles/7c15436666e89cb6c194