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AnnData Example (https://anndata.readthedocs.io/ based on https://anndata-tutorials.readthedocs.io/en/latest/getting-started.html)
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import numpy as np | |
import pandas as pd | |
import anndata as ad | |
# Generate example data and build AnnData object | |
vals = np.random.rand(5, 10).astype('f') | |
adata = ad.AnnData(vals) | |
adata | |
adata.X | |
adata.obs_names = [f"cell_{i:d}" for i in range(adata.n_obs)] | |
adata.var_names = [f"gene_symbol_{i:d}" for i in range(adata.n_vars)] | |
print(adata.obs_names[:3]) | |
tmp = np.random.choice(["cell1", "cell2", "cell3"], size=(adata.n_obs,)) | |
adata.obs["cell_type1_id"] = tmp | |
adata.obs | |
tmp = np.random.choice(["cell_type1", "cell_type2", "cell_type3"], size=(adata.n_obs,)) | |
adata.obs["cell_type2_id"] = tmp | |
adata.obs | |
tmp = np.random.choice(["G1", "G2", "G3"], size=(adata.n_vars,)) | |
adata.var["gene_type1_id"] = tmp | |
adata.var | |
tmp = np.random.choice(["gene_type1", "gene_type2", "gene_type3"], size=(adata.n_vars,)) | |
adata.var["gene_type2_id"] = tmp | |
adata.var | |
bdata = adata[adata.obs.cell_type1_id == "cell1"] | |
bdata | |
# Write tables | |
adata.write_csvs("output", skip_data=False) | |
# Write h5ad format | |
adata.write(filename="anndata.h5ad") | |
# Read data | |
cdata = ad.read_h5ad(filename="anndata.h5ad") | |
cdata |
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cell_type1_id | cell_type2_id | ||
---|---|---|---|
cell_0 | cell1 | cell_type3 | |
cell_1 | cell3 | cell_type1 | |
cell_2 | cell3 | cell_type2 | |
cell_3 | cell1 | cell_type3 | |
cell_4 | cell3 | cell_type1 |
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gene_type1_id | gene_type2_id | ||
---|---|---|---|
gene_symbol_0 | G1 | gene_type2 | |
gene_symbol_1 | G1 | gene_type3 | |
gene_symbol_2 | G1 | gene_type1 | |
gene_symbol_3 | G1 | gene_type2 | |
gene_symbol_4 | G2 | gene_type3 | |
gene_symbol_5 | G2 | gene_type2 | |
gene_symbol_6 | G1 | gene_type2 | |
gene_symbol_7 | G2 | gene_type2 | |
gene_symbol_8 | G3 | gene_type3 | |
gene_symbol_9 | G2 | gene_type2 |
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0.19100112 | 0.46828908 | 0.8881515 | 0.3272082 | 0.44101298 | 0.13238761 | 0.06625041 | 0.42884535 | 0.45125055 | 0.54849786 | |
---|---|---|---|---|---|---|---|---|---|---|
0.73450327 | 0.968179 | 0.44648758 | 0.10997138 | 0.2849175 | 0.5890882 | 0.053468592 | 0.7258484 | 0.8558861 | 0.28162476 | |
0.34389842 | 0.6496306 | 0.70850253 | 0.5590548 | 0.93613046 | 0.18099453 | 0.2924698 | 0.13112783 | 0.25727338 | 0.29037902 | |
0.67264825 | 0.36550987 | 0.7641897 | 0.5965308 | 0.30332556 | 0.7546757 | 0.88458216 | 0.6731449 | 0.1464198 | 0.2685193 | |
0.59329206 | 0.8943774 | 0.061935168 | 0.20078066 | 0.16109781 | 0.0445861 | 0.456919 | 0.34288594 | 0.4962387 | 0.3457089 |
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