Created
April 21, 2015 11:39
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ImageJ macro to split LSM files
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| sourcedir=getDirectory("select source directory with lsm files"); | |
| destdir=getDirectory("select or create destination directory"); | |
| list = getFileList(sourcedir); | |
| Dialog.create("New LSM Batch color converter options"); | |
| Dialog.addChoice("Export format ", newArray("Tiff", "Jpeg")); | |
| Dialog.addCheckbox("Output merged channels image(s) ", true); | |
| Dialog.addCheckbox("Process all time points ", false); | |
| Dialog.addCheckbox("Process all slices ", false); | |
| Dialog.addCheckbox("Convert to 8-bit ", false); | |
| Dialog.show(); | |
| type = Dialog.getChoice(); | |
| merge_image = Dialog.getCheckbox(); | |
| process_frames = Dialog.getCheckbox(); | |
| process_slices = Dialog.getCheckbox(); | |
| convert8 = Dialog.getCheckbox(); | |
| for (i=0; i<list.length; i++) { | |
| path = sourcedir+list[i]; | |
| if (endsWith(path, ".lsm")) { | |
| run("LSM Reader", "open=["+path+"]"); | |
| processImage(); | |
| close(); | |
| } | |
| } | |
| function processImage() { | |
| run("Make Composite", "composite"); | |
| id=getImageID; | |
| t=getTitle(); | |
| t = replace(t,".","_"); | |
| t = replace(t,":","_"); | |
| t = replace(t," ","_"); | |
| Stack.getDimensions(width, height, channels, slices, frames); | |
| ones=""; while(lengthOf(ones)<channels) ones+="1"; | |
| if (process_frames==false) frames=1; | |
| if (process_slices==false) slices=1; | |
| for (f=1;f<= frames;f++) { | |
| Stack.setFrame(f); | |
| for (s=1;s<= slices;s++) { | |
| Stack.setSlice(s); | |
| for (c=1;c<= channels;c++) { | |
| selectImage(id); | |
| Stack.setChannel(c); | |
| run("Duplicate...", "title="+t+"_T"+f+"_Z"+s+"_"+getInfo('slice.label')); | |
| if (convert8==true) run ("8-bit"); | |
| saveImage(); | |
| } | |
| if (merge_image==true) { | |
| selectImage(id); | |
| Stack.setDisplayMode("composite"); | |
| Stack.setActiveChannels(ones); | |
| run("RGB Color", " keep"); | |
| rename(t+"_T"+f+"_Z"+s+"_Merge"); | |
| saveImage(); | |
| } | |
| } | |
| } | |
| } | |
| function saveImage() { | |
| saveAs(type,destdir+getTitle); | |
| close(); | |
| } |
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